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KMID : 0903619820230040284
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Journal of the Korean Society for Horticultural Science
1982 Volume.23 No. 4 p.284 ~ p.288
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Humidity and Tissues for in vitro Pollen Germination and Tube Elongation
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이수성/Lee, S S
Ferrari, T E/Wallace, D H
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Abstract
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Pollen germination of 5 Brossica species was studied with relative humedities (RH) from 7% to 100%. RH at 98% was best for pollen germination on glass slides and surfaces of plant tissues including leaf, leaf petiole, peduncle, filament, sepal, petal and pistil. After germination, pollen tube elongation occurred only on the surface of filaments and at the base of petals, where the epidermal cells were long and $quot;cigar-shaped$quot;. Tube elongation was unidirectional, along the longitudinal axis of these long subjacent epidermal cells. Use of this in vitro system as a bioassay for studying stages of pollen germination. and lobo orientation is discussed.
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